The Fair Trade Commission (FTC) of South Korea has launched an investigation into whether Google obstructed Samsung's development of its homegrown Tizen platform.
Google is no stranger to an antitrust investigation, and this latest probe focuses on Mobile Application Distribution Agreement (MADA) which Google and Samsung both signed back in 2011.
Under this agreement, Samsung is forced to ensure its Android smartphones use Google as the default search engine, and that the firm's apps - including Gmail, YouTube and Google Play - come pre-installed on devices as standard.
The two firms also signed an anti-fragmentation agreement, which blocks Samsung from developing its own Android-based operating system - despite the likes of Amazon and Xiaomi having built their own platforms using the Android Open Source Project (AOSP).
This has raised eyebrows in South Korea and the FTC has said that the agreements may have forced Samsung to take a reserved approach to the development of its own Tizen platform, which the firm was forced to create as incompatible with the Android app catalogue.
"We are currently checking if Google thwarted competition in the OS market," an FTC official told the Korea Times.
The FTC is also re-opening an investigation from 2013, which saw it probe whether MADA was harmful to domestic search providers such as Naver and Daum.
While Google was cleared back then because official determined its competitors market shares were not being significantly affected, changing market dynamics have apparently convinced regulators to take a fresh look at the situation.
A Google Korea spokesman said: "Android is an open source platform. Our partner agreements are entirely voluntary — anyone can use Android without Google.
"The Android OS can be downloaded for free. It can be modified and used to build a phone. Many companies have used Android's source code as the starting point for their own operating systems."
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