The US government has made it a federal crime to take a Galaxy Note 7 on an aircraft, and anyone who does so risks a £148,000 fine.
The new rules were set out by the Federal Aviation Administration (FAA) over the weekend as part of an Emergency Restriction/Prohibition Order.
The rules ban passengers carrying the smartphone "on their person, in carry-on baggage, in checked baggage, or as cargo". Anyone who inadvertently brings one on a plane must power it down immediately.
Carriers are also required to "deny boarding to a passenger in possession" of the phone, the FAA said.
The US Department of Transportation has also chimed in and said that anyone caught attempting to travel with the phone will have it confiscated and may face fines up to $179,933 (around £148,000).
"We recognise that banning these phones from airlines will inconvenience some passengers, but the safety of all those aboard an aircraft must take priority," said US transportation secretary Anthony Foxx.
"We are taking this additional step because even one fire incident in-flight poses a high risk of severe personal injury and puts many lives at risk.”
The US Consumer Product Safety Commission (CPSC), which ordered a second recall of the Galaxy Note 7 last week, stated that there have been nearly 100 reports of batteries in Note 7 phones overheating in the US.
"The fire hazard with the original Note 7 and with the replacement Note 7 is simply too great for anyone to risk it and not respond to this official recall," said CPSC chairman Elliot Kaye.
"I would like to remind consumers once again to take advantage of the remedies offered, including a full refund. It’s the right thing to do and the safest thing to do."
This dramatic step comes just days after Samsung was dealt a major blow with the news that 30 per cent of Note 7 owners plan to switch to an iPhone.
Eight per cent said that they will pick up a Google Pixel smartphone, while the remaining 62 per cent said that they will go with another Android handset. Some 40 per cent of those quizzed said that they will never buy another Samsung smartphone.
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